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KMID : 0894520100140040215
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Development & Reproduction
2010 Volume.14 No. 4 p.215 ~ p.223
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A Current Advance of Gene Targeting and Gene Trapping Methods As Tools of Making Transgenic Mice
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Kang Hae-Mook
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Abstract
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The construction of transgenic mouse using embryonic stem (ES) cells has been crucial in the functional studies of gene on mouse genome. Gene knockout mice have been powerful for elucidating the function of genes as well as a research model for human diseases. Gene targeting and gene trapping mathods have been the representative technologies for making the knockout mice by using ES cells. Since the gene targeting and the gene trapping methods were independently developed about 20 years ago, it"s efficiency and productivity has been improved with a advance of molecular biology. Conventional gene targeting method has been changes to high throughput conditional gene targeting. The combination of the advantage of gene targeting and gene tapping elements allows to extend a spectrum of gene trapping and to improve the efficiency of gene targeting. These advance should be able to produce the mutant with various phenotype to target a certain gene, and in postgenome era they have served as crucial research tools in understanding the functional study of whole genome in mouse.
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KEYWORD
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Transgenic mouse, Gene targeting, Gene trapping, Insertional mutagenesis, Embryonic stem(ES) cells
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